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fc 131 1096 superscript vilo cdna synthesis kit thermo fisher  (Illumina Inc)


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    Illumina Inc fc 131 1096 superscript vilo cdna synthesis kit thermo fisher
    Fc 131 1096 Superscript Vilo Cdna Synthesis Kit Thermo Fisher, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fc+131+1096+superscript+vilo+cdna+synthesis+kit+thermo+fisher/Nextera+XT+DNA+Library+Preparation+Kit/pmc09112118__NIHMS1667151___supplement___4-421-112-110
    Average 99 stars, based on 11844 article reviews
    fc 131 1096 superscript vilo cdna synthesis kit thermo fisher - by Bioz Stars, 2026-10
    99/100 stars

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    Protease Inhibitor:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    DNA Library Preparation:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    cDNA Synthesis:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    SYBR Green Assay:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Polymerase Chain Reaction:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Imaging:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Transfection:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Virus:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Immunodetection:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    ChIP-qPCR:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Magnetic Beads:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Chromatin Immunoprecipitation:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Microarray:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Quantitative RT-PCR:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Cloning:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS

    Recombinant:

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2

    Article Title: FOS licenses early events in stem cell activation driving skeletal muscle regeneration
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Polybrene MilliporeSigma Cat# TR-1003 Puromycin MilliporeSigma Cat# P9620 Hygromycin B solution Millipore Sigma Cat# H0654 Doxycycline Hyclate Epic Pharma Cat# NDC 42806312-05 0.5M EDTA, pH 8.0 Millipore Sigma Cat# 03690 0.5M EGTA, pH 8.0 Boston Bioproducts Cat# BM-151 Cardiotoxin (CTX) Latoxan Cat# L8102 HEPES Millipore Sigma Cat# H3375 NaCl Millipore Sigma Cat# 71376 Formaldehyde Solution, 37% Millipore Sigma Cat# F8775 Glycine Millipore Sigma Cat# 50046 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Millipore Sigma Cat# 11836170001 Critical commercial assays GeneChIP Mouse Gene 1.0 ST Array Thermo Fisher Cat# 901168 SMART-Seq v4 Ultra Low Input RNA Kit Takara (CloneTech) Cat# 634890 Nextera XT DNA Library Preparation Kit Illumina Cat# FC-131-1096 SuperScript VILO cDNA Synthesis Kit Thermo Fisher Cat# 1175050 RNeasy Micro Kit QIAGEN Cat# 74004 Power SYBR Green PCR Master Mix Thermo Fisher Cat# 4367659 FastStart Universal SYBR Green Master Mix (Rox) Sigma-Aldrich Cat# 4913850001 Click-it EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# C10337 Trans-IT-LT1 Transfection Reagent Mirus Cat# Mir2304 Lipofectamine 2000 Thermo Fisher Cat# 11668030 Lenti-X Virus concentrator Kit Takara (Clonetech) Cat# 631232 Vector M.O.M Immunodetection Kit Vector Labs Cat# BMK-2202 Gateway LR Clonase II Enzyme Mix Thermo Fisher Cat# 11791100 iDeal ChIP-qPCR Kit x 24 Diagenode Cat# C01010180 DiaMAG protein A-coated magnetic beads (ChIP-Seq-grade) Diagenode Cat# C03010020 Deposited data Raw and analyzed RNA-Seq Data This study GEO: GSE119895 Raw and analyzed Microarray Data This study GEO: GSE119695 Experimental models: organisms/strains Mouse: C57BL/6J Jackson Labs Cat# 000664 Mouse: C57BL/10ScSn-Dmdmdx/J Jackson Labs Cat# 001801 Mouse: B6.Cg-Tg(Fos/EGFP)1-3Brth/J Jackson Labs Cat# 014135 Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J Jackson Labs Cat# 007909 Mouse: B6.Cg-Pax7 tm1(cre/ERT2)Gaka/J Jackson Labs Cat# 017763 Mouse: Fosfl/fl (C57BL/6J) Fleischmann et al., 2003 N/A Mouse: C57BL/Ka- b-actin-EGFP Wright et al., 2001 N/A Oligonucleotides Primers for qRT-PCR and cloning, Table S4 This study N/A Recombinant DNA pMD2.G N/A Gift from Didier Trono, Addgene #12259 psPAX2 N/A Gift from Didier Trono, Addgene #12260 (Continued on next page) Cell Reports 34, 108656, January 26, 2021 e2 Article ll OPEN ACCESS



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